Effect of Dietary Supplementation with Dried Olive Leaf Powder and L-Carnitine on Hematological and Biochemical Blood Parameters of Broiler Chickens (Ross 308)

Natural feed additives have received increasing attention in
poultry nutrition due to their potential role in improving physiological status
and reducing metabolic disorders in broiler chickens. Olive leaves are rich in
phenolic compounds and antioxidants, while L-carnitine plays an essential
role in fatty acid metabolism and energy production. This study was
conducted at the broiler chicken farm of the Animal Production Unit, College
of Medicinal and Industrial Plants, University of Kirkuk, Iraq, from 9
September 2024 to 13 October 2023. A total of 180 broiler chicks (Ross 308)
were used in the experiment. At 7 days of age, the birds were randomly
allocated to six experimental treatments with three replicates each: T1
(control), T2 (1 g/kg dried olive leaf powder), T3 (2 g/kg dried olive leaf
powder), T4 (500 mg/kg L-carnitine), T5 (1 g/kg dried olive leaf powder + 500
mg/kg L-carnitine), and T6 (2 g/kg dried olive leaf powder + 500 mg/kg L
carnitine). Birds were fed a starter diet until 21 days of age followed by a
grower diet until 35 days, with feed and water provided ad libitum. The
results showed that dietary supplementation with dried olive leaf powder
and L-carnitine, either individually or in combination, significantly
improved several hematological and biochemical blood parameters
compared with the control treatment. The supplemented treatments showed
increased concentrations of total protein, albumin, and high-density
lipoprotein (HDL), along with significant reductions in total cholesterol,
triglycerides, low-density lipoprotein (LDL), and very low-density
lipoprotein (VLDL). In addition, the activities of liver enzymes aspartate
aminotransferase (AST) and alanine aminotransferase (ALT) were reduced,
indicating improved liver function and metabolic status. Moreover,
lymphocyte percentage and the heterophil/lymphocyte (H/L) ratio were not
negatively affected. Dietary supplementation with dried olive leaf powder
and L-carnitine can improve biochemical blood parameters and metabolic
health in broiler chickens (Ross 308), suggesting that these additives may
serve as effective natural feed supplements in broiler production systems.