Effect of Growth Regulators and AgNO3 NPs on the Leaves Extract of Stevia rebaudiana in the Production of Bioactive Compounds in Vitro

The experiment was conducted in the graduate laboratory, Department of biology, College of Science, Al-Qadisiyah University, to study the effect of different concentrations of Clorx on sterilizing explanted on the growth response , and study of the effect of different levels of plant hormones and AgNO3 NPs on the production of active compounds. Used concentrations of (8, 10, and 12%) of Clorx in sterilization explanted where grown in MS medium at a concentration of 4.44 g with sucrose 30 g and agar 7 g and incubated in the growth room at a temperature of 25 °C. The results were recorded after 40 days. The results showed that the response rate of the growths was higher at a concentration of 10%, and this percentage was ideal for sterilization, as the response rate was recorded. 72%, while the contamination rate in the samples was 12 %, while the chlorine concentration was 8%, it recorded a growth rate of 78%, but the contamination rate was high if it was recorded at 28%, while the concentration was 12%, the growth response rate was low, as it was recorded at 48% and the contamination rate was 10%. Plant Growth Regulators and AgNO3 nanoparticles were added to the medium at different concentrations. The results of the GC-MS analysis showed a group of compounds of medical importance, including n-Hexadecanoic acid, Octadecanoic acid, Cycloheptasiloxane, Diethyl Phthalate, Phytol, and Propanoic acid. The The compounds n-Hexadecanoic acid, Octadecanoic Acid, and Cycloheptasiloxane showed the highest ratio in the treatment (4 NAA, 2 Kin, 3 AgNO3 NPs), while the compound Diethyl Phthalate achieved the highest ratio in the treatment (2 NAA, 1 Kin, 6 AgNO3 NPs). The compound Phytol showed the highest ratio in the treatment (2 NAA, 1 Kin, 0 AgNO3 NPs), and the highest ratio for the compound Propanoic acid was observed in the treatment (4 NAA, 2 Kin, 6 AgNO3 NPs).