Study of the Level of Enzyme Muramidase and Some Biochemical Variable and Finding the Relationship between Them in Patients with Crohn`S Disease
Downloads
Crohn’s disease is a chronic inflammatory condition affecting any part of the gastrointestinal tract, causing symptoms like abdominal pain and diarrhea. It affects millions worldwide, with higher prevalence in North America and Europe and its incidence continues to rise globally.
Aim of study: The main objective of the current research study the relationship between specific biochemical parameters and Crohn` s disease in Mosul City. A cross study in a group of 70 as 48 males and 22 females participants with crohn` s disease and 70 healthy peoples 30 males, 40 females. Healthy participants by paid a visit outpatient clinics and private hospitals in Mosul city from the date of 19/1 to 25/2 of 2025. Whole blood samples were collected from all volunteers' patients and healthy to be analyze for serum Mur activity, TNF-α, MDA, GPx, PN and CAT. The results were showed that the majority patients with Crohn` s disease indicated higher levels of serum Mur activity in compared to healthy controls Also patients with Crohn` s disease having an increase of TNF, MDA, GPx, PN, CAT and decreases in levels of Fe,Se and Mn in patients with Crohn s disease.
1. Al-Abachi, S. Z., Yaseen, S. M., & Shihab, G. A. (2022). Biochemical study of consumption Zahdi dates (Phoenix dactylifera) in type 2 diabetic patients. Rafidain Journal of Science, 31(1), 11-22. https://doi.org/10.33899/rjs.2022.172924
2. Alam, M. S., Khan, M. A., & Rahman, M. M. (2019). Evaluation of oxidative stress markers in patients with chronic inflammatory diseases. J. Clin. Biochem. Nutr., 64(2), 120–127. https://doi.org/10.3164/jcbn.18-74
3. Al-Hakeem, Q. H. Y., & Al-Sadoon, M. B. H. (2024). Effect liposome Nanoparticles Loaded with Natural Products Isolated from Commiphora myrrh on Plasminogen Activator Inhibitor-1 in Mice Induced Aortic Atherosclerosis. Egyptian Journal of Veterinary Sciences, 55(6), 1737–1749. https://ejvs.journals.ekb.eg/article_344173.html
4. Al-Taii, R. A., & Jankeer, M. H. (2025). Evaluation of the role of some myokines and tumor necrosis factor-alpha in women with polycystic ovary syndrome in Mosul City-Iraq. Rafidain Journal of Science, 34(2), 23-30. https://doi.org/10.33899/rjs.2025.187739
5. Baumgart, D. C., & Sandborn, W. J. (2012). Crohn’s disease. The Lancet, 380(9853), 1590–1605. https://doi.org/10.1016/S0140-6736(12)60026-9
6. Gálvez-Iriqui, A. C., Plascencia-Jatomea, M., & Bautista-Baños, S. (2020). Lysozymes: Characteristics, mechanism of action and technological applications on the control of pathogenic microorganisms. Revista Mexicana de Fitopatología, 38(3), 360–383. https://doi.org/10.18781/r.mex.fit.2005-6
7. Huang, Z., Rose, A.H., & Hoffmann, P.R. (2012). Selenium in inflammation. Antioxid. Redox Signal., 16(7), 705–743.
8. Khor, B., Gardet, A., & Xavier, R. J. (2011). Genetics and pathogenesis of inflammatory bowel disease. Nature, 474(7351), 307–317. https://doi.org/10.1038/nature10209
9. Klebanoff, S. J. (2005). Myeloperoxidase: friend and foe. Journal of Leukocyte Biology, 77(5), 598–625. https://doi.org/10.1189/jlb.0605325
10. Liu, J., et al. (2021). Peroxynitrite in health and disease: Molecular mechanisms and clinical implications. Free Radical Biology and Medicine, 174, 54–68. https://doi.org/10.1016/j.freeradbiomed.2021.07.024
11. Mayo Clinic. (2024, October 29). Crohn’s disease – Symptoms and causes. Mayo Clinic. https://www.mayoclinic.org/diseases-conditions/crohns-disease/symptoms-causes/syc-20353304
12. Nawaz, N., Wen, S., Wang, F., Nawaz, S., Raza, J., Iftikhar, M., & Usman, M. (2022). Lysozyme and its application as antibacterial agent in food industry. Molecules, 27(19), 6305. https://doi.org/10.3390/molecules27196305
13. Pruzanski, W., Marcon, N., Ottaway, C., & Prokipchuk, E. (1977). Muramidase (lysozyme) in Crohn's disease and in ulcerative colitis. The American Journal of Digestive Diseases, 22(11), 995–998. https://doi.org/10.1007/BF01076199
14. Rezaie, A., Parker, R.D., & Abdollahi, M. (2007). Oxidative stress in IBD. Dig. Dis. Sci., 52(9): 2015–2021. https://doi.org/10.1007/s10620-006-9581-5
15. Russell, R. K., Satsangi, J., & Donnelly, L. A. (2011). Genetics of Crohn’s disease: Beyond NOD2. Inflammatory Bowel Diseases, 17(12), 2351–2360. Retrieved from https://journals.lww.com/ibdjournal/Fulltext/2011/12000/Genetics_of_Crohn_s_Disease__Beyond_NOD2.17.aspx
16. Szczeklik, K., Krzysciak, W., Domagala-Rodacka, R., Mach, P., Darczuk, D., Cibor, D., Pytko-Polonczyk, J., Rodacki, T., & Owczarek, D. (2016). Alterations in glutathione peroxidase and superoxide dismutase activities in plasma and saliva in relation to disease activity in patients with Crohn's disease. Journal of Physiology and Pharmacology, 67(5), 709–715. Retrieved from http://www.jpp.krakow.pl
17. Walfish, A. E., & Companioni, R. A. C. (2025, January). Crohn disease (Regional enteritis; granulomatous ileitis; granulomatous ileocolitis). In M. Nguyen (Reviewer), Merck Manuals Professional Version. Retrieved July 23, 2025, from
18. Widbom, L., Ekblom, K., Karling, P., & Hultdin, J. (2020). Patients developing inflammatory bowel disease have iron deficiency and lower plasma ferritin years before diagnosis: A nested case-control study. European Journal of Gastroenterology & Hepatology, 32(9), 1147–1153. https://pubmed.ncbi.nlm.nih.gov/32541236/
19. Yan, W., Meihao, W., Zihan, S., Lingjie, H., Haotian, C., Qian, C., & Lianli, S. (2022). Correlation between Crohn's disease activity and serum selenium concentration. Clinical Therapeutics, 44(5), 736-743.e3. https://doi.org/10.1016/j.clinthera.2022.04.008
20. Yaseen, A. T., & Saeed, M. K. (2021). Evaluation the levels of some Mineral Elements in Patients with Stroke. Egyptian Journal of Chemistry, 64(12), 7113-7116. https://doi.org/10.21608/ejchem.2021.80149.3956.
