Estradiol-Induced Testicular Toxic Alterations in Wister Rats During Adolescence: Anatomical and Histological Investigation
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The present investigation was conducted to characterize the structural susceptibility of the male gonadal tissue to exogenous estrogen exposure during the pubertal window, with particular emphasis on morphological regression and histological remodeling induced by estradiol benzoate administration. Methods: twenty male rats at two months aged were split equally into two groups:(control, and treated). The treatment rats were given sub\cutaneous injections of 60 μg/ of rat oestradiol benzoate dissolved in 0.26 ml of olive oil for 15 consecutive days; while control rats were given subcutaneous injections of 0.26 ml of olive oil. Twenty-four hours following final treatment dose, Rats in both groups were given general anesthesia and weighed., had their scrotums dissected and had their testicles separated from other organs. Weight, length, width, and volume calculated. For histological examination, sample from testis were kept in 10% natural formalin. Using hematoxylin and eosin (H&E) staining, identify general architecture and histopathological changes. A unique stain called Masson's trichrome staining is used to detect collagen fibres. The results showing the testicular morphometric measurements in control rats were similarly to intact; while all data showed a marked decrease in treatment rats (G2). Histological analyses showed that control rat testis in with full structural and histological integrity; while estradiol treated rats testis presenting significantly decreased in diameter and epithelial height of seminiferous tubules (p≤0.05) and there were histological alterations, including irregular, atrophy of seminiferous tubules, and loss of organized stratified epithelium. There was no evidence of typical Leydig cells. This research determined that observed changes clearly demonstrate how estradiol impacted the histological makeup and reproductive capabilities of testis, leading to reduced spermatogenesis and diminished secretory function of interstitial cells. Consequently, overall findings affirm rats subjected 60 μg of estradiol benzoate each rat. during their puberty stage exhibited toxic effecting on testis, ultimately resulting in decreased male fertility.
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