Inflammatory and Regenerative Responses of the Epididymis Following Local Anesthesia: An Immunohistochemical Study
Downloads
This experimental study evaluated immunohistochemical changes in epididymal tissue following different methods of local anesthesia using lidocaine and novocaine. A white outbred rat model was used with markers of inflammation (CD45) and regenerative potential (CD56). Intratesticular administration was associated with increased inflammatory activity (↑CD45) and reduced regenerative capacity (↓CD56), with the most pronounced effects observed after novocaine injection. In contrast, conduction anesthesia, particularly with lidocaine, showed lower CD45 expression and relative preservation of CD56-positive cells. A combined approach (lidocaine conduction anesthesia with microdose intratesticular novocaine) reduced inflammation while maintaining regenerative potential. These findings demonstrate that the route of anesthetic administration critically influences the immunomorphological response of epididymal tissue and support the use of less traumatic combined protocols in scrotal interventions.
Sh. A. Karimov, Fundamentals of Histology and Embryology. Tashkent, Uzbekistan: Fan Publishing, 2018, pp. 112–130.
M. T. Rakhimov and D. S. Yusupov, “Morphological changes in male reproductive organs under pharmacological exposure,” Journal of Medical Sciences of Uzbekistan, vol. 5, no. 2, pp. 45–52, 2020.
A. K. Tashpulatov, “Immunohistochemical analysis of tissue reactions in experimental conditions,” Uzbek Biological Journal, vol. 3, no. 1, pp. 22–29, 2019.
B. N. Mamatkulov, Pathological Anatomy. Tashkent, Uzbekistan: Ibn Sino Publishing, 2017, pp. 210–225.
S. R. Kadirov and U. B. Akhmedov, “Effects of local anesthetics on tissue regeneration,” Central Asian Medical Review, vol. 4, no. 3, pp. 60–66, 2021.
D. E. Norboev, “Inflammatory responses in reproductive tissues: Experimental study,” Bulletin of Tashkent Medical Academy, vol. 6, no. 2, pp. 75–81, 2020.
F. A. Abdurakhmanov, Human Anatomy. Tashkent, Uzbekistan: Yangi Asr Avlodi, 2016, pp. 98–115.
N. S. Pulatova and R. K. Ismailov, “Cellular mechanisms of inflammation and regeneration,” Uzbek Journal of Clinical Research, vol. 2, no. 4, pp. 33–40, 2022.
J. E. Hall, Guyton and Hall Textbook of Medical Physiology, 14th ed. Philadelphia, USA: Elsevier, 2021, pp. 1010–1025.
V. Kumar, A. K. Abbas, and J. C. Aster, Robbins and Cotran Pathologic Basis of Disease, 10th ed. Philadelphia, USA: Elsevier, 2020, pp. 45–70.
L. Mescher, Junqueira’s Basic Histology: Text and Atlas, 15th ed. New York, USA: McGraw-Hill, 2018, pp. 210–230.
P. R. Wheater, Wheater’s Functional Histology, 6th ed. Philadelphia, USA: Elsevier, 2014, pp. 150–165.
A. Stevens and J. Lowe, Human Histology, 4th ed. Philadelphia, USA: Elsevier, 2015, pp. 190–205.
R. S. Cotran, V. Kumar, and T. Collins, Robbins Pathologic Basis of Disease, 9th ed. Philadelphia, USA: Elsevier, 2015, pp. 60–75.
S. B. Yuldashev, “Immunohistochemical markers in reproductive system pathology,” Uzbek Medical Journal, vol. 7, no. 1, pp. 55–61, 2023.
