Assessment of Several Newcastle Disease Vaccines' Immunological Effectiveness in Broiler Chicks with Challenge
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125 one-day-old chicks were obtained from the Barakat Al-Zahra nursery in Diwaniyah city and randomly assigned to five groups of identical size. Groups A, B, C, D, and E received vaccinations. with several strains included (HB1, Lasota, Clone-30, KomarovR, VG/GA, F) on days 1 & 9 of age, but group E was deemed a control group because it had not received any vaccine. Each group's birds had their blood samples obtained. 13 and 23 day of age. Average antibody titers of NDV Hem agglutination inhibition test (HI) and immunosorbant assay (ELIZA) testing following primary and secondary vaccinations. The five groups were exposed to a virulent strain of NDV (Kudu strain 113), 22 day of age by intramuscular injection. The results showed a significant superiority (P 0.05) in the level of antibodies measured in the Hem agglutination inhibition (HI) test and the ELISA test for the group vaccinated with the mesogenic (KomarovR) by injection method and that preceded by a live attenuated vaccine given by spray method. The same group showed very mild disease symptoms that disappeared later, and this group did not record mortality at 0/25 followed by group which vaccinated by live attenuated vaccine by drinking water preceded by dropping. In same time the infection rate increased significantly in the control group following the challenge, this group showed a significant decrease (P 0.05) in antibodies compared to the rest of the groups when this standard was measured at the ages of 13 and 23 days of the birds.
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